Our peptides are shipped in lyophilized (freeze-dried) powder form. Lyophilization is a process in which water is removed from the peptide after it is frozen and placed under a vacuum, causing the ice to change directly from a solid to vapor state without passing through a liquid phase.
Before lyophilized peptides can be utilized in the lab, they must be reconstituted; meaning, they must be dissolved in a liquid solution.
The most commonly used item for reconstitution is bacteriostatic water.
Example using bacteriostatic as the diluent:
Remove the plastic cap from the peptide vial.
Remove the plastic cap from the bacteriostatic water vial.
To prevent bacterial contamination, wipe the rubber area of the vials with an alcohol swab.
Extract 1mL (milliliters) of water from the bacteriostatic water vial.
Insert the 1mL (milliliters) of bacteriostatic water into the peptide vial, letting the water slowly enter the vial.
Lightly swirl the solution until all peptide is dissolved — do not shake the vial.
Final concentration = peptide mass ÷ diluent volume
