Our peptides are shipped in lyophilized (freeze-dried) powder form. Lyophilization is a process in which water is removed from the peptide after it is frozen and placed under a vacuum, causing the ice to change directly from a solid to vapor state without passing through a liquid phase.
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Before lyophilized peptides can be utilized in the lab, they must be reconstituted; meaning, they must be dissolved in a liquid solution.
The most commonly used item for reconstitution is bacteriostatic water.
Example using bacteriostatic as the diluent:
Step 1 – Remove the plastic cap from the peptide vial
Step 2 – Remove the plastic cap from the bacteriostatic water vial
Step 3 – To prevent bacterial contamination,wipe the rubber area of the vials with an alcohol swab.
Step 4 – Extract 1mL (milliliters) of water from the bacteriostatic water vial.
Step 5 – Insert the 1mL (milliliters) of bacteriostatic water into the peptide vial, letting the water slowly enter the vial.
Step 6 – Lightly swirl the solution until all peptide is dissolved – do not shake the vial.
Example solutions
5mg of peptide with 1mL water = 0.5mg/500mcg per 0.1mL/10IU
5mg of peptide with 2mL water = 0.25mg/250mcg per 0.1mL/10IU
5mg of peptide with 3mL water = 0.17mg/17mcg per 0.1mL/10IU
2mg of peptide with 1mL water = 0.2mg/200mcg per 0.1mL/10IU
2mg of peptide with 2mL water = 0.1mg/100mcg per 0.1mL/10IU
2mg of peptide with 3mL water = 0.067mg/67mcg per 0.1mL/10IU