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Research Guide

Peptide Reconstitution

Our peptides are shipped in lyophilized (freeze-dried) powder form. Lyophilization is a process in which water is removed from the peptide after it is frozen and placed under a vacuum, causing the ice to change directly from a solid to vapor state without passing through a liquid phase.

Before lyophilized peptides can be utilized in the lab, they must be reconstituted; meaning, they must be dissolved in a liquid solution.

The most commonly used item for reconstitution is bacteriostatic water.

Example using bacteriostatic as the diluent:

Reconstitution Steps

1

Remove the plastic cap from the peptide vial.

2

Remove the plastic cap from the bacteriostatic water vial.

3

To prevent bacterial contamination, wipe the rubber area of the vials with an alcohol swab.

4

Extract 1mL (milliliters) of water from the bacteriostatic water vial.

5

Insert the 1mL (milliliters) of bacteriostatic water into the peptide vial, letting the water slowly enter the vial.

6

Lightly swirl the solution until all peptide is dissolved — do not shake the vial.

Example Solutions

2mg Peptide
1mL water 0.2mg / 200mcg per 0.1mL / 10IU
2mL water 0.1mg / 100mcg per 0.1mL / 10IU
3mL water 0.067mg / 67mcg per 0.1mL / 10IU
5mg Peptide
1mL water 0.5mg / 500mcg per 0.1mL / 10IU
2mL water 0.25mg / 250mcg per 0.1mL / 10IU
3mL water 0.17mg / 17mcg per 0.1mL / 10IU
10mg Peptide
1mL water 1mg / mcg per 0.1mL / 10IU
2mL water 0.5mg / mcg per 0.1mL / 10IU
3mL water 0.33mg / mcg per 0.1mL / 10IU