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Laboratory Tool · Research Use Only

Peptide Concentration Calculator

Concentration and dilution arithmetic for preparing laboratory solutions from lyophilized research materials. For research use only.

For laboratory research use only. This calculator performs general concentration and dilution arithmetic for preparing laboratory solutions from lyophilized research materials. Products sold by Rocky Mountain Peptides are not for human or veterinary use.

Step 1

Stock solution concentration

Enter the amount of material in the vial and the volume of diluent added to it. The calculator returns the concentration of the resulting stock solution.

mg
mL
Concentration5 mg/mL
Concentration5,000 µg/mL
Per 1 µL5 µg/µL

Amount of material in common pipette volumes

VolumeAmount (µg)Amount (mg)

Step 2

Dilution to a working concentration (C1V1 = C2V2)

Calculate how much stock solution to transfer, and how much diluent to add, to prepare a working solution at a lower concentration.

C1 × V1 = C2 × V2  →  V1 = (C2 × V2) ÷ C1
mg/mL
mg/mL
mL
Stock to transfer (V1)100 µL
Diluent to add900 µL
Dilution factor1:10

How the math works

  • Concentration (mg/mL) = amount of material (mg) ÷ volume of diluent (mL).
  • 1 mg/mL = 1,000 µg/mL = 1 µg/µL.
  • Amount in a volume = concentration × volume. For example, 100 µL of a 5 mg/mL solution contains 0.5 mg (500 µg).
  • Dilution uses C1V1 = C2V2: the amount of material is the same before and after adding diluent.

Results assume the full stated amount of material dissolves completely and that the added diluent volume equals the final solution volume. Actual concentration can differ: lyophilized peptides may be supplied as salt forms (such as acetate or trifluoroacetate), and net peptide content can be lower than the gross vial weight. Check the certificate of analysis for your lot where available.

Related: Reconstitution guide · Storage guide · Molecular weight chart · Glossary

This tool is provided for general laboratory arithmetic only. Rocky Mountain Peptides products are intended solely for in-vitro research and laboratory use. They are not drugs, foods, cosmetics or supplements and are not for human or veterinary use.